PHYLOGENY

red_bullet.gif (914 bytes) Phylip on the Web (L'Institute Pasteur, France) - for information on this set of programs see: Joseph Felenstein, (University of Washington, U.S.A.).

red_bullet.gif (914 bytes) T-Rex (Tree and reticulogram REConstruction) - is dedicated to the reconstruction of phylogenetic trees, reticulation networks and to the inference of horizontal gene transfer (HGT) events. T-REX includes several popular bioinformatics applications such as MUSCLE, MAFFT, Neighbor Joining, NINJA, BioNJ, PhyML, RAxML, random phylogenetic tree generator and some well-known sequence-to-distance transformation models. It also comprises fast and effective methods for inferring phylogenetic trees from complete and incomplete distance matrices as well as for reconstructing reticulograms and HGT networks (Reference: Alix, C. et al. 2012.  Nucl. Acids Res. 40 (W1): W573-W579).

red_bullet.gif (914 bytes) Phylogeny.fr - is a simple to use web service dedicated to reconstructing and analysing phylogenetic relationships between molecular sequences.It includes multiple alignment (MUSCLE, T-Coffee, ClustalW, ProbCons), phylogeny (PhyML, MrBayes, TNT, BioNJ), tree viewer (Drawgram, Drawtree, ATV) and utility programs (e.g. Gblocks to eliminate poorly aligned positions and divergent regions) (Reference: A. Dereeper  et al,. 2008. Nucl. Acids Res. 36 (Web Server Issue):W465-9).

red_bullet.gif (914 bytes) PHYML - is a simple, fast and accurate algorithm to estimate maximum likelihood phylogenies from DNA and protein sequences. This tool provides the user with a number of options, e.g. nonparametric bootstrap and estimation of various evolutionary parameters. (Reference:  Guindon, S.et al. 2010. Systematic Biology, 59(3):307-21).
ProtTest (David Posada, University of Vigo, Spain) - estimates the empirical model of aminoacid substitution that fits the data best among 64 candidate models. PROTTEST calculates AIC, AICc and BIC values, and obtain a rank of model fits, model-averaged parameter estimates, or measures of parameter importance.  Mac OSX, Windows and Linux versions are available for downloading.

 Phylemon2 - a suite of web-tools for molecular evolution, phylogenetics and phylogenomics (Reference:Sánchez, R. et al. 2011.Nucl. Acids Res. 39/suppl_2/W470)

POWER (PhylOgenetic Web Repeater) - allows users to carry out phylogenetic analysis on most programs of PHYLIP package repeatedly. POWER provide two pipelines to process the analysis. One of them includes multiple sequence alignment (MSA) at the beginning of the pipeline whereas the other begin phylogenetic analysis with aligned sequence. Very user friendly. (Reference: C.-Y. Lin. et al. 2005. Nucl. Acids Res. 33: W553-W556).
red_bullet.gif (914 bytes) Phylodendron - phylogenetic tree printer (D.G. Gilbert, Indiana Univ.) - very useful in visualizing *.dnd file from aligments and saving the results as .GIF, .PS or .PDF files.  N.B. The font style and size can be altered in the .PDF output format.
red_bullet.gif (914 bytes) Phylogenetic tree prediction - GeneBee service (Belozersky Institute of Physico-chemical Biology, Moscow State University, Russia)

red_bullet.gif (914 bytes) CVTree - Composition Vector - constructs whole-genome based phylogenetic trees without sequence alignment by using a Composition Vector (CV) approach. It was first developed to infer evolutionary relatedness of microbial organisms and then successfully applied to viruses, chloroplasts, and fungi. It circumvents the ambiguity of choosing the genes for phylogenetic reconstruction and avoids the necessity of aligning sequences of essentially different length and gene content. (Reference: Z. Xu & B. Hao 2009. Nucl. Acids Res. 37(Web Server issue:W174-W178)

red_bullet.gif (914 bytes) webPRANKincorporates phylogeny-aware multiple sequence alignment, visualisation and post-processing in an easy-to-use web interface.(Reference: Löytynoja, A., & Goldman, N. 2010. BMC Bioinformatics. 11:579).

red_bullet.gif (914 bytes) ReplacementMatrix - maximum-likelihood estimation of amino acid replacement rate matrices.(Reference: Dang, C.C. et al. 2011. Bioinformatics. 27(19):2758-2760).

red_bullet.gif (914 bytes) DIVEIN - starting with a set of aligned sequences, DIVEIN estimates evolutionary parameters and phylogenetic trees while allowing the user to choose from a variety of evolutionary models; it then reconstructs the consensus (CON), most recent common ancestor (MRCA), and center of tree (COT) sequences. DIVEIN also provides tools for further analyses. (Reference: Deng, W. et al. 2010. Biotechniques. 48(5):405-408).

red_bullet.gif (914 bytes) Ribosomal RNA Analysis - The Ribosomal Database Project II (Michigan State University Centre for Microbial Ecology, U.S.A.).
red_bullet.gif (914 bytes) Ridom - Ribosomal RNA analysis for clinically relevant bacteria - (University of Würzburg, Germany)
red_bullet.gif (914 bytes) Rifle - (Universitat Bielefeld, Germany) The RIFLE system compares restriction patterns of 16S rDNA amplicons against a database of theoretical restriction patterns generated from a 16S rDNA database

Molecular Taxonomy

red_bullet.gif (914 bytes) AmphoraNet - is capable of assigning a probability-weighted taxonomic group for each phylogenetic marker gene found in the input metagenomic sample; the webserver is based on the AMPHORA2 workflow.  It uses 31 bacterial and 104 archaeal protein coding marker genes for metagenomic phylotyping. Most of these are single copy genes, therefore AmphoraNet is suitable for estimating the taxonomic composition of bacterial and archaeal communities from metagenomic shotgun sequencing data. (Reference: Kerepesi, C. et al. 2014. Gene 533: 538–540)

red_bullet.gif (914 bytes) GGDC - Genome-to-Genome Distance Calculator - The species concept for Bacteria and Archaea is ultimately based on DNA-DNA hybridization (DDH). This web service can be used for genome-based species delineation with complete or incomplete genomes sequences. The server calculate intergenomic distances; and, these are converted into similarity values analogous to DDH and sent to you via e-mail. (Reference: Meier-Kolthoff, J.P. et al. 2013. BMC Bioinformatics 14:60).

red_bullet.gif (914 bytes) MyTaxa - represents a new algorithm that extends the Average Amino Acid Identity (AAI) concept to identify the taxonomic affiliation of a query genome sequence or a sequence of a contig assembled from a metagenome, including short sequences, and to classify sequences representing novel taxa at three levels  i.e., species, genus and phylum. MyTaxa can assign a larger number of sequences and with higher accuracy compared to other tools available for the same purposes. This is largely attributed to the fact that MyTaxa considers all genes present in an unknown (query) sequence as classifiers and quantifies the classifying power of each gene using predetermined weights, which are derived from the analysis of orthologs of the gene from all available complete genomes. (Reference: Luo, C. et al. 2014. Nucl. Acids Res).

red_bullet.gif (914 bytes) ANI calculatorestimates the average nucleotide identity using both best hits (one-way ANI) and reciprocal best hits (two-way ANI) between two genomic datasets. Typically, the ANI values between genomes of the same species are above 95% while values below 75% are not to be trusted, and AAI should be used instead. This tool supports both complete and draft genomes (multi-fasta).(Reference: Goris J. et al. 2007. Int J Syst Evol Microbiol. 57:81-91).