Accuracy of Online ORF Prediction Programs

Using the genome of coliphage HK97 (NC_002167) the following programs were tested for their accuracy in identifying ORFs correctly.

GenBank - lists 60 genes (ignoring the frameshift)

Program	Number of Genes Identified	Correct Assignment of Gene Termini		Overall % Correct
		Number with correct 5' ends	Number with correct 3' ends
EasyGene	41	39	40	65
Glimmer (Pathogene)	54	38	43	63
FgenesB	53	41	48	68
GeneHacker	35	27	31	45
GeneMark.hmm	55	43	47	72
Generation	29	23	25	38
Glimmer (OakRidge)	62	31	37	52