ONLINE ANALYSIS TOOLS
(INTERNET RESOURCES for MOLECULAR BIOLOGISTS)
Analysis of nucleotide and protein sequence data was initially restricted to those with access to complicated mainframe or expensive desktop computer programs (for example PC/GENE, Lasergene, MacVector, Accelrys etc.). The availability of online tools permits even the novice molecular biologist the opportunity to derive a considerable amount of useful nformation from nucleotide or protein sequence data. For those with no experience I have provided three sequences: (a) a DNA sequence, (b) a protein sequence, and (c) four protein sequences presented in FASTA format. Prior to trying out a Web Site select the sequence and copy to clipboard. Each of the items in blue text is hyperlinked to a site on the Web. Each of these Web Sites has a box into which you can "Paste" your sequence. Click on the button labeled "Search," "Run" or "Submit." If in doubt use the default setting that the sites provide, but for the more adventuresome some of the sites offer the chance of modifying the search strategy.
ONLINE RESOURCES (tutorials and glossaries)
CONVERT SEQUENCE (upper to low case, complement, reverse, RNA to DNA)
MELTING TEMPERATURE (Tm)
PCR PRIMER DESIGN
REPEATS & SECONDARY STRUCTURE
RESTRICTION ENDONUCLEASE SITES
TRANSLATION (EUKARYOTIC GENES) (identification of introns and exons)
WINDOWS- & JAVA or PERL-BASED PROGRAMS - free molecular biology programs
The Bio-Web: Resources for Molecular and Cell Biologists is a non-commercial, educational site with the only purpose of facilitating access to biology-related information over the internet.
CREATED BY: Dr. Andrew Kropinski, Departments of Food Science, Molecular & Cellular Biology; and, Pathobiology University of Guelph, Guelph , Ontario, N1G 2W1 CANADA
Problems and suggestions: Contact - Andrew M. Kropinski (Email: Phage.Canada (at) gmail.com)